Laboratory equipment for through passing continuous cultivation of microbes.

Three Decades of Continuous Records for Biophysical Chemistry Laboratory (BCL)

The Biophysical Chemistry Laboratory (BCL) was established in 1986 at Institute of Biochemistry and Biophysics (IBB), University of Tehran as the main base and the mother of biophysical chemistry in Iran. BCL is a famous worldwide research lab in the area of thermodynamics of protein denaturation and biomacromolecular interaction. The first student that joined BCL was MSc student in 1987, while...

Standardization of laboratory equipment.

New strategies for cultivation and detection of previously uncultured microbes.

An integrative approach was used to obtain pure cultures of previously uncultivated members of the divisions Acidobacteria and Verrucomicrobia from agricultural soil and from the guts of wood-feeding termites. Some elements of the cultivation procedure included the following: the use of agar media with little or no added nutrients; relatively long periods of incubation (more than 30 days); prot...

Development ofbioconsortia for optimizing nutrient supplementation through microbes for sustainable tobacco production

Increased interests in low-input agriculture in recent years has seen the growing developmentin the use of commercial biological inoculants to increase the mobilization of key nutrients suchas nitrogen (N), phosphorus (P) and potassium (K) to enhance their availability to crop plants.The objectives of this field experiment with tobacco were to determine i) reduced rates ofinorganic fertilizer c...

Detection of airborne microbes in a composting facility by cultivation based and cultivation-independent methods.

Standard methods for quantification of airborne bacteria are based on cultivation and counting of grown colonies. From complex natural environments it is known that only a small fraction of the total number of cells can be cultivated on routinely used agar-media. Direct microscopic cell counting after DNA-staining usually generates higher concentrations of one to two magnitudes. The objective o...